登入 臺大生技所
| 年度 | 2022 |
|---|---|
| 全部作者 | 宋麗英 ,Keppeke GD, Chang CC, Antos CL, Peng M, Sung LY, Coelho Andrade LE, Liu JL. |
| 論文名稱 | IMPDH forms the cytoophidium in zebrafish. |
| 期刊名稱 | Developmental Biology |
| 期數 | 478 |
| 頁碼 | 89-101 |
| 總頁數 | 12 |
| 發表日期 | 2021-10-01 |
| 摘要 | Inosine monophosphate dehydrogenase (IMPDH) catalyzes the rate-limiting step in de novo guanine nucleotide biosynthesis. Its activity is negatively regulated by the binding of GTP. IMPDH can form a membraneless subcellular structure termed the cytoophidium in response to certain changes in the metabolic status of the cell. The polymeric form of IMPDH, which is the subunit of the cytoophidium, has been shown to be more resistant to the inhibition by GTP at physiological concentrations, implying a functional correlation between cytoophidium formation and the upregulation of GTP biosynthesis. Herein we demonstrate that zebrafish IMPDH1b and IMPDH2 isoforms can assemble abundant cytoophidium in most of cultured cells under stimuli, while zebrafish IMPDH1a shows distinctive properties of forming the cytoophidium in different cell types. Point mutations that disrupt cytoophidium structure in mammalian models also prevent the aggregation of zebrafish IMPDHs. In addition, we discover the presence of the IMPDH cytoophidium in various tissues of larval and adult fish under normal growth conditions. Our results reveal that polymerization and cytoophidium assembly of IMPDH can be a regulatory machinery conserved among vertebrates, and with specific physiological purposes. |
| 語言 | 中文 |
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